|Application ||WB, IF|
|Calculated MW||52258 Da|
|Other Names||Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5|
|Target/Specificity||A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human Smad5. This antibody detects Smad5 phosphorylated at Serine 463 and 465.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Smad5 Antibody Phospho (pS463/465) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).|
|Cellular Location||Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4|
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Provided below are standard protocols that you may find useful for product applications.
Smad5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad5 is directly activated by BMP type Ia or Ib receptors upon BMP-2 stimulation and transduced BMP-2 signals to the nucleus by forming a complex with Smad 4, activating the transcription of the target gene in the nucleus (2-3). BMP activates Smad5 by phosphorylation at Ser463 and Ser465, which are the equivalent phosphorylation sites for Smad 1 and Smad 8.
1. Eppert et al. Cell 86: 543-552, 1996
3. Abdollah, S., Mac
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