Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Transcription factor PU.1 Antibody   

Transcription factor PU.1 Antibody

Rabbit Monoclonal Antibody

  • WB - Transcription factor PU.1 Antibody AJ1789a
    A. Western blot analysis on (A) U937 and (B) TF-1 cell lysates using anti-Trancription factor PU.1 RabMAb (Cat. #AJ1789a), dilution 1:1000.
  • IHC - Transcription factor PU.1 Antibody AJ1789a
    B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Transcription factor PU.1 RabMAb (Cat. #AJ1789a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P17947
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR3159Y
Calculated MW 31083 Da
Gene ID 6688
Other Names Transcription factor PU1, 31 kDa-transforming protein, SPI1
Target/Specificity A synthetic peptide corresponding to residues on the C terminus of transcription factor PU.1 was used as an immunogen.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTranscription factor PU.1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPI1
Function Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00237, ECO:0000269|PubMed:23166356}
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


Transcription factor PU.1 is a member of the Ets family of transcription factors and is required for the development of multiple hematopoietic lineages (3). It plays a pivotal role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development. Transcription factor PU.1 and AML1, a DNA-binding subunit of the CBF transcription factor family, act as tumor suppressors in leukemia. The function of PU.1 is down-regulated by AML1-ETO in myeloid leukemia, whereas overexpression of PU.1 restores normal differentiation (1-2). The lack of transcription factor PU.1 protein expression in classical Hodgkin?s disease (cHD), a lymphoproliferative disease of predominantly B-cell origin, likely contributes to the lack of immunoglobulin expression and incomplete B-cell phenotype characteristic of the Reed-Sternberg cells in cHD (4).


1. Huang G, et al. Nat Genet. 40(1):51-60, 2008
2. Vangala R K, et al. Blood 101(1):270-277, 2003
3. Kim H, et al. Blood 104(13):3894-3900, 2004
4. Torlakovic E, et al. American Journal of Pathology 159:1807-1814, 2001

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

Cat# AJ1789a
Alternative Products:

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions