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Ubiquitin Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - Ubiquitin Antibody (C-term) AJ1807a
    A. Western blot analysis on Hela cell lysate using anti-Ubiquitin (C-term) RabMAb (Cat. #AJ1807a), dilution 1:2,000.
  • IHC - Ubiquitin Antibody (C-term) AJ1807a
    B. Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using anti-Ubiquitin (C-term) RabMAb (Cat. #AJ1807a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P62988
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP296Y
Other Names UBC, UBCEP2, Ubiquitin
Target/Specificity A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen.
Dilution WB~~1:1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUbiquitin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)

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Ubiquitin (originally ubiquitous imunopoeitic polypeptide-UBIP) is an 8.5 kDa protein highly conserved among eukaryotic species (1). Ubiquitin acts through its post-translational attachment (ubiquitinylation) to other proteins, where these modifications alter the function, location or trafficking of the protein and targets it for destruction by the 26S proteasome. Ubiquitinylation also termed ubiquination, is an ATP-dependent process that involves the action of at least three enzymes: E1 (activating enzyme), E2 (conjugation enzyme) and E3 (ligase) working sequentially (2-4). This process is an important regulatory tool that controls the concentration of key signaling and regulatory proteins as well as removing damaged, misfolded or mutant proteins (5).


1. Goldstein,G., Scheid, M., Hamerling, U., Boyse, E.A., Schlesinger, D.H. & Niall, D. (1975) Proc. Natl. Acad. Sci. USA 72 1-15.
2. Hatfield, P.M. & Vierstra, R.D. (1992) J. Biol. Chem. 267 14799-14803.
3. Sullivan, M.L. & Vierstra, R.D. (1991) J. Biol. Chem. 266 23878-23885.
4. Gonen, H., et al. (1996) J Biol Chem 271 302-310.
5. Hatakeyama S. , Nakayama K.I. Biochem. Biophys. Res. Commun. 302: 635-645 (2003).

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Cat# AJ1807a

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