|Reactivity||Human, Monkey, Dog|
|Dilution||IHC-P (20 µg/ml)|
|Other Names||High affinity cGMP-specific 3', 5'-cyclic phosphodiesterase 9A, 220.127.116.11, PDE9A|
|Target/Specificity||Human PDE9A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.|
|Reconstitution & Storage||Long term: -70°C; Short term: +4°C|
|Precautions||PDE9A Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP (PubMed:9624146, PubMed:18757755, PubMed:21483814). Specifically regulates natriuretic-peptide- dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart (PubMed:25799991). Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory (By similarity).|
|Cellular Location||Isoform PDE9A1: Cell projection, ruffle membrane. Cytoplasm, perinuclear region. Golgi apparatus. Endoplasmic reticulum. Cell membrane, sarcolemma Isoform PDE9A3: Cytoplasm. Endoplasmic reticulum|
|Tissue Location||Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood (PubMed:9624146). Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate (PubMed:12565835). In brain, present in the cortex, cerebellum, and subiculum (at protein level) (PubMed:22328573). In heart, primarily localizes to myocytes (PubMed:25799991)|
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Provided below are standard protocols that you may find useful for product applications.
Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.
Fisher D.A.,et al.J. Biol. Chem. 273:15559-15564(1998).
Guipponi M.,et al.Hum. Genet. 103:386-392(1998).
Rentero C.,et al.Biochem. Biophys. Res. Commun. 301:686-692(2003).
Wang P.,et al.Gene 314:15-27(2003).
Rentero C.,et al.BMC Mol. Biol. 7:39-39(2006).
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