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Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| IHC-P |
|---|---|
| Primary Accession | Q9H4A3 |
| Reactivity | Human, Mouse, Rabbit, Hamster, Monkey, Horse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 251kDa |
| Dilution | IHC-P (5-7.5 µg/ml) |
| Gene ID | 65125 |
|---|---|
| Other Names | Serine/threonine-protein kinase WNK1, 2.7.11.1, Erythrocyte 65 kDa protein, p65, Kinase deficient protein, Protein kinase lysine-deficient 1, Protein kinase with no lysine 1, hWNK1, WNK1, HSN2, KDP, KIAA0344, PRKWNK1 |
| Target/Specificity | Human WNK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
| Reconstitution & Storage | Long term: -70°C; Short term: +4°C |
| Precautions | WNK1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | WNK1 (HGNC:14540) |
|---|---|
| Function | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide- sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron. |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide- sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L.
References
Verissimo F.,et al.Oncogene 20:5562-5569(2001).
Vidal-Petiot E.,et al.PLoS ONE 7:E37751-E37751(2012).
Scherer S.E.,et al.Nature 440:346-351(2006).
Moore T.M.,et al.J. Biol. Chem. 275:4311-4322(2000).
Nagase T.,et al.DNA Res. 4:141-150(1997).
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