|Dilution||IHC-P (2.5 µg/ml)|
|Other Names||Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, Solute carrier family 1 member 3, SLC1A3, EAAT1, GLAST, GLAST1|
|Target/Specificity||Human SLC1A3 / GLAST. BLAST analysis of the peptide immunogen showed no homology with other human proteins.|
|Reconstitution & Storage||Long term: -70°C; Short term: +4°C|
|Precautions||SLC1A3 / EAAT1 Antibody (Cytoplasmic Domain) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7521911, PubMed:8123008, PubMed:20477940, PubMed:26690923, PubMed:28032905, PubMed:28424515). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:20477940). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:20477940). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity).|
|Cellular Location||Cell membrane; Multi- pass membrane protein|
|Tissue Location||Detected in brain (PubMed:8218410, PubMed:7521911, PubMed:8123008). Detected at very much lower levels in heart, lung, placenta and skeletal muscle (PubMed:7521911, PubMed:8123008). Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia (PubMed:7521911).|
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Provided below are standard protocols that you may find useful for product applications.
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Shashidharan P.,et al.Biochim. Biophys. Acta 1216:161-164(1993).
Arriza J.L.,et al.J. Neurosci. 14:5559-5569(1994).
Kawakami H.,et al.Biochem. Biophys. Res. Commun. 199:171-176(1994).
Stoffel W.,et al.FEBS Lett. 386:189-193(1996).
Vallejo-Illarramendi A.,et al.J. Neurochem. 95:341-348(2005).
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