|Application ||WB, IHC-P, IF|
|Dilution||IHC-P (5 µg/ml), WB (0.5-2 µg/ml),|
|Other Names||Caspase recruitment domain-containing protein 11, CARD-containing MAGUK protein 1, Carma 1, CARD11, CARMA1|
|Target/Specificity||synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||CARMA1 / CARD11 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the costimulatory signal essential for T- cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. Also activates the TORC1 signaling pathway.|
|Cellular Location||Cytoplasm. Membrane raft. Note=Colocalized with DPP4 in membrane rafts.|
|Tissue Location||Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL- 60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells|
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Provided below are standard protocols that you may find useful for product applications.
Involved in the costimulatory signal essential for T- cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
Bertin J.,et al.J. Biol. Chem. 276:11877-11882(2001).
Hillier L.W.,et al.Nature 424:157-164(2003).
Scherer S.W.,et al.Science 300:767-772(2003).
Gaide O.,et al.FEBS Lett. 496:121-127(2001).
Gaide O.,et al.FEBS Lett. 505:198-198(2001).
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