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Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, ICC |
|---|---|
| Primary Accession | Q8WXG6 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 183kDa |
| Dilution | IHC-P (2.5 µg/ml), WB (1:250-1:500), |
| Gene ID | 8567 |
|---|---|
| Other Names | MAP kinase-activating death domain protein, Differentially expressed in normal and neoplastic cells, Insulinoma glucagonoma clone 20, Rab3 GDP/GTP exchange factor, MADD {ECO:0000312|EMBL:AAB57735.1, ECO:0000312|HGNC:HGNC:6766} |
| Target/Specificity | peptide corresponding to amino acids near the carboxy terminus of human MADD |
| Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. |
| Precautions | DENN / MADD Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MADD {ECO:0000312|EMBL:AAB57735.1, ECO:0000312|HGNC:HGNC:6766} |
|---|---|
| Function | Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect. |
| Cellular Location | Membrane. Cytoplasm |
| Tissue Location | Highly expressed in fetal brain and kidney; adult testis, ovary, brain and heart. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Isoform 5 shows increased cell proliferation and isoform 2 shows decreased. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis); isoform 1 is susceptible to inducing apoptosis, isoform 5 is resistant and isoform 3 and isoform 4 have no effect.
References
Chow V.T.K.,et al.DNA Seq. 6:263-273(1996).
Schievella A.R.,et al.J. Biol. Chem. 272:12069-12075(1997).
Chow V.T.K.,et al.Genome 41:543-552(1998).
Al-Zoubi A.M.,et al.J. Biol. Chem. 276:47202-47211(2001).
Nagase T.,et al.DNA Res. 4:141-150(1997).
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