|Application ||WB, IHC-P, IF|
|Dilution||IF (5 µg/ml), IHC-P (5 µg/ml), WB (0.5-1 µg/ml)|
|Other Names||Barrier-to-autointegration factor, Breakpoint cluster region protein 1, Barrier-to-autointegration factor, N-terminally processed, BANF1, BAF, BCRG1|
|Target/Specificity||15 amino acid peptide from near the carboxy terminus of human BANF1.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||BANF1 / BAF / BCRP1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging.|
|Cellular Location||Barrier-to-autointegration factor: Nucleus Cytoplasm. Chromosome. Nucleus envelope. Note=Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase The phosphorylated form (by VRK1 or vaccinia virus B1 kinase) shows a cytoplasmic localization whereas the unphosphorylated form locates almost exclusively in the nucleus (PubMed:24600006, PubMed:16495336). May be included in HIV-1 virions via its interaction with viral GAG polyprotein|
|Tissue Location||Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes|
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Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
Lee M.S.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:1528-1533(1998).
Lynch R.A.,et al.Genomics 52:17-26(1998).
Zhang J.,et al.Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Harris D.,et al.J. Biol. Chem. 275:39671-39677(2000).
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