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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   CD177 Antibody (clone MEM-166)   

CD177 Antibody (clone MEM-166)

Mouse Monoclonal Antibody

  • IHC - CD177 Antibody (clone MEM-166) ALS12023
    Anti-CD177 antibody IHC of human intravascular neutrophils.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IP, Flo
Primary Accession Q8N6Q3
Reactivity Human, Monkey
Host Mouse
Clonality Monoclonal
Clone Names MEM-166
Calculated MW 46kDa
Dilution Flo (1 µg/10E6 cells), IHC-P (10 µg/ml),
Additional Information
Gene ID 57126
Other Names CD177 antigen, Human neutrophil alloantigen 2a, HNA-2a, NB1 glycoprotein, NB1 GP, Polycythemia rubra vera protein 1, PRV-1, CD177, CD177, NB1, PRV1
Target/Specificity Human granulocytes
Reconstitution & Storage +4°C. Store undiluted.
PrecautionsCD177 Antibody (clone MEM-166) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD177 (HGNC:30072)
Function In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369).
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor Membrane raft; Lipid-anchor, GPI- like-anchor. Secreted Cytoplasmic granule membrane. Cell projection, lamellipodium. Note=Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (PubMed:17244676, PubMed:17580308). In neutrophils, stored predominantly in secondary and tertiary granules (PubMed:18462208). Can also be shedded from the cell membrane (PubMed:12239154, PubMed:18462208) Localizes to lamellar protrusions in spreading neutrophils (PubMed:28807980).
Tissue Location Highly expressed in normal bone marrow and weakly expressed in fetal liver (PubMed:10753836). During neutrophil differentiation, expression begins at the metamyelocyte stage and continues throughout the subsequent stages (at protein level) (PubMed:17244676, PubMed:18462208, PubMed:24926686) Expressed by a subset of mature neutrophils (at protein level) (PubMed:10753836, PubMed:28240246, PubMed:12377969, PubMed:18462208, PubMed:12675722, PubMed:17244676, PubMed:17580308, PubMed:21193407, PubMed:24926686, PubMed:28807980, PubMed:27227454). The percentage of neutrophils expressing CD177 varies across the population (PubMed:17244676, PubMed:27227454). Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET) (PubMed:10753836, PubMed:12377969).
Research Areas
Citations (0)

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Temerinac S.,et al.Blood 95:2569-2576(2000).
Kissel K.,et al.Eur. J. Immunol. 31:1301-1309(2001).
Kissel K.,et al.Blood 99:4231-4233(2002).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

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$ 425.00
Cat# ALS12023
Availability: 5 days
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