|Application ||WB, IHC-P, ICC, E|
|Dilution||IHC-P (5 µg/ml), WB (0.5-1 µg/ml),|
|Other Names||Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6-alpha, Processed cyclic AMP-dependent transcription factor ATF-6 alpha, ATF6|
|Target/Specificity||17 amino acid peptide from near the amino terminus of human ATF6|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||ATF6 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'- ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type II membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.
Zhu C.,et al.Mol. Cell. Biol. 17:4957-4966(1997).
Yoshida H.,et al.J. Biol. Chem. 273:33741-33749(1998).
Gregory S.G.,et al.Nature 441:315-321(2006).
Hai T.,et al.Genes Dev. 3:2083-2090(1989).
Hai T.,et al.Genes Dev. 4:682-682(1990).
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