|Application ||WB, IHC-P, E, IP|
|Dilution||IHC-P (5-10 µg/ml)|
|Other Names||TFIIH basal transcription factor complex helicase XPB subunit, 220.127.116.11, Basic transcription factor 2 89 kDa subunit, BTF2 p89, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH 89 kDa subunit, TFIIH p89, Xeroderma pigmentosum group B-complementing protein, ERCC3, XPB, XPBC|
|Target/Specificity||Recognizes an epitope located between aa242-261 of ERCC-3 (largest subunit of TFIIH).|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||ERCC3 / XPB Antibody (aa242-261, clone 3G4) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP- dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.|
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Provided below are standard protocols that you may find useful for product applications.
ATP-dependent 3'-5' DNA helicase, component of the core- TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage.
Weeda G.,et al.Mol. Cell. Biol. 10:2570-2581(1990).
Weeda G.,et al.Cell 62:777-791(1990).
Weeda G.,et al.Nucleic Acids Res. 19:6301-6308(1991).
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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