|Application ||WB, IHC-P, IF|
|Dilution||IHC-P (1:100), WB (1:500-1:1000),|
|Other Names||Ran GTPase-activating protein 1, RanGAP1, RANGAP1, KIAA1835, SD|
|Target/Specificity||Reacts with human & Xenopus RanGAP|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||RANGAP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTPase activator for RAN (PubMed:8146159, PubMed:8896452, PubMed:16428860). Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed:8896452, PubMed:27160050). Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed:27160050).|
|Cellular Location||Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic during interphase Detected at the nuclear envelope during interphase (PubMed:11854305, PubMed:15037602). Targeted to the nuclear pores after sumoylation (PubMed:11854305). During mitosis, associates with mitotic spindles, but is essentially not detected at the spindle poles (PubMed:11854305, PubMed:15037602). Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase (PubMed:11854305). Mitotic location also requires sumoylation (PubMed:11854305)|
|Tissue Location||Highly expressed in brain, thymus and testis.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.
Bischoff F.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:1749-1753(1995).
Nagase T.,et al.DNA Res. 8:85-95(2001).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Dunham I.,et al.Nature 402:489-495(1999).
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