|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Sheep, Horse, Bovine, Dog|
|Dilution||ELISA (1:32000), IHC-P (2.5 µg/ml), WB (0.1-0.3 µg/ml)|
|Other Names||Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, FOXO3, FKHRL1, FOXO3A|
|Target/Specificity||Human FOXO3 / FOXO3A. Both variants represent identical protein (NP_001446.1and NP_963853.1).|
|Reconstitution & Storage||Store at -20°C. Minimize freezing and thawing.|
|Precautions||FOXO3 / FOXO3A Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, PubMed:16751106). Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' (PubMed:21329882). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301).|
|Cellular Location||Cytoplasm, cytosol. Nucleus. Mitochondrion matrix Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note=Retention in the cytoplasm contributes to its inactivation (PubMed:10102273, PubMed:15084260, PubMed:16751106). Translocates to the nucleus upon oxidative stress and in the absence of survival factors (PubMed:10102273, PubMed:16751106). Translocates in a AMPK- dependent manner into the mitochondrion in response to metabolic stress (PubMed:23283301, PubMed:29445193)|
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Provided below are standard protocols that you may find useful for product applications.
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
Anderson M.J.,et al.Genomics 47:187-199(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Hillion J.,et al.Blood 90:3714-3719(1997).
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