|Application ||WB, IHC-P, E|
|Dilution||ELISA (1:10000), IHC-P (1:200), WB (1:500-1:2000)|
|Other Names||Lysine--tRNA ligase, 220.127.116.11, Lysyl-tRNA synthetase, LysRS, KARS, KIAA0070|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||KARS Antibody (clone 8G12C1) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739).|
|Cellular Location||Isoform Cytoplasmic: Cytoplasm, cytosol Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted. Note=Secretion is induced by TNF- alpha (PubMed:15851690). Cytosolic in quiescent mast cells Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739)|
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Provided below are standard protocols that you may find useful for product applications.
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
Shiba K.,et al.J. Biol. Chem. 272:22809-22816(1997).
Tolkunova E.,et al.J. Biol. Chem. 275:35063-35069(2000).
Nomura N.,et al.DNA Res. 1:223-229(1994).
Martin J.,et al.Nature 432:988-994(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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