|Application ||WB, IHC-P|
|Calculated MW||50228 Da|
|Dilution||IHC-P (5 - 10 µg/ml), WB|
|Other Names||RUVBL1, 49 kDa TBP-interacting protein, ECP54, INO80 complex subunit H, INO80H, Nuclear matrix protein 238, Pontin52, RuvB-like 1, TIH1, TAP54-alpha, TIP49, NMP 238, PONTIN, RuvB (E coli homolog)-like 1, RuvB-like 1 (E. coli), ECP-54, NMP238, Pontin ...|
|Target/Specificity||Human TIP49 / RUVBL1|
|Reconstitution & Storage||PBS, 0.05% sodium azide. Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
|Precautions||Anti-TIP49 / RUVBL1 Antibody (aa1-268) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||INO80H, NMP238, TIP49, TIP49A|
|Function||Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. May be able to bind plasminogen at cell surface and enhance plasminogen activation.|
|Cellular Location||Nucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells|
|Tissue Location||Ubiquitously expressed with high expression in heart, skeletal muscle and testis|
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