|Application ||WB, IHC-P|
|Calculated MW||86397 Da|
|Dilution||IHC-P (1:100), WB (1:500 - 1:1000)|
|Other Names||SMO, Frizzled family member 11, FZD11, Gx, Protein Gx, SMOH, Smoothened, Smoothened homolog|
|Target/Specificity||Recognizes endogenous levels of Smoothened protein.|
|Reconstitution & Storage||PBS, pH 7.3, 0.01% sodium azide, 30% glycerol. Store at -20°C. Aliquot to avoid freeze/thaw cycles.|
|Precautions||Anti-SMO / Smoothened Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed:19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).|
|Cellular Location||Membrane; Multi-pass membrane protein. Cell projection, cilium|
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