|Application ||WB, IHC-P|
|Calculated MW||38044 Da|
|Dilution||IHC-P (10 µg/ml), WB (0.5 - 1 µg/ml)|
|Other Names||DNAJB1, DnaJ protein homolog 1, DNAJ1, HDj-1, Hdj1, Heat shock 40 kDa protein 1, Hsp40, HSPF1, RSPH16B, Sis1, Radial spoke 16 homolog B, Heat shock 40kD protein 1, Heat shock protein 40, Human DnaJ protein 1|
|Target/Specificity||Endogenous levels of human, and mouse HSP40 protein. Sequence homology predicts that it will also react with rat and bovine HSP40 protein.|
|Reconstitution & Storage||Lyophilized from PBS, pH 7.4, 0.02% sodium azide. Store lyophilized at -20°C. The reconstituted product can be stored for short term at 4 °C or long term at -20 °C or below. Avoid freeze/thaw cycles.|
|Precautions||Anti-DNAJB1 / Hsp40 Antibody (aa170-220) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DNAJ1, HDJ1, HSPF1|
|Function||Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).|
|Cellular Location||Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock|
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