|Application ||WB, IHC-P, IF|
|Predicted||Human, Mouse, Rat|
|Calculated MW||98809 Da|
|Dilution||IF (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000)|
|Other Names||POLK, DINP, DNA polymerase kappa, DINB1, DINB protein|
|Target/Specificity||Human POLK / DNA Polymerase Kappa|
|Reconstitution & Storage||Affinity purified|
|Precautions||Anti-POLK / DNA Polymerase Kappa Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.|
|Cellular Location||Nucleus. Note=Detected throughout the nucleus and at replication foci (PubMed:12414988). Recruited to DNA damage sites in response to ultraviolet irradiation: N6-methyladenosine (m6A)-containing mRNAs accumulate in the vicinity of DNA damage sites and their presence is required to recruit POLK (PubMed:28297716).|
|Tissue Location||Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.|
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