|Application ||WB, E|
|Calculated MW||12580 Da|
|Other Names||Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1|
|Target/Specificity||This EIF4EBP1 monoclonal antibody is generated from mouse immunized with EIF4EBP1 recombinant protein.|
|Format||Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EIF4EBP1 Antibody (ascites) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq].
She, Q.B., et al. Cancer Cell 18(1):39-51(2010)
Aoyagi, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(6):2640-2645(2010)
Naukkarinen, J., et al. PLoS Genet. 6 (6), E1000976 (2010) :
Kumar, A., et al. PLoS ONE 5 (1), E8730 (2010) :
Villalonga, P., et al. J. Biol. Chem. 284(51):35287-35296(2009)
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