Mouse Monoclonal Antibody (Mab)
|Application ||WB, E|
|Calculated MW||35609 Da|
|Other Names||NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI|
|Target/Specificity||Purified His-tagged NFKBIA protein was used to produced this monoclonal antibody.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NFKBIA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||IKBA, MAD3, NFKBI|
|Function||Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.|
|Cellular Location||Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.|
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Provided below are standard protocols that you may find useful for product applications.
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Huxford T., et al. Cell 95:759-770(1998).
Cockman M.E., et al. Proc. Natl. Acad. Sci. U.S.A. 103:14767-14772(2006).
Haskill S., et al. Cell 65:1281-1289(1991).
Jungnickel B., et al. J. Exp. Med. 191:395-402(2000).
Liu B., et al. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
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