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KARS Antibody

Purified Mouse Monoclonal Antibody

  • WB - KARS Antibody AO1195a
    Figure 1: Western blot analysis using KARS mouse mAb against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (3).
  • IHC - KARS Antibody AO1195a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human cervical carcinoma, showing cytoplasmic localization with DAB staining using KARS mouse mAb.
  • IHC - KARS Antibody AO1195a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using KARS mouse mAb
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15046
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8G12C1
Isotype IgG1
Calculated MW 68048 Da
Description KARS: lysyl-tRNA synthetase. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.
Immunogen Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3735
Other Names Lysine--tRNA ligase,, Lysyl-tRNA synthetase, LysRS, KARS, KIAA0070
Dilution WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKARS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA0070
Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739).
Cellular Location Isoform Cytoplasmic: Cytoplasm, cytosol Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted. Note=Secretion is induced by TNF- alpha (PubMed:15851690). Cytosolic in quiescent mast cells Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739)
Research Areas
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1. J Biol Chem. 2000 Nov 10;275(45):35063-9. 2. J Virol. 2001 Jun;75(11):5043-8. 3. Biochem Biophys Res Commun. 2002 Feb 15;291(1):158-64. 4. Cell. 2004 Oct 15;119(2):195-208.

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$ 325.00
Cat# AO1195a
Availability: 7-10 days
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