|Application ||WB, IHC, E|
|Calculated MW||68048 Da|
|Description||KARS: lysyl-tRNA synthetase. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.|
|Immunogen||Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli. |
|Formulation||Ascitic fluid containing 0.03% sodium azide. |
|Other Names||Lysine--tRNA ligase, 188.8.131.52, Lysyl-tRNA synthetase, LysRS, KARS, KIAA0070|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/500 - 1/2000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KARS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739).|
|Cellular Location||Isoform Cytoplasmic: Cytoplasm, cytosol Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted. Note=Secretion is induced by TNF- alpha (PubMed:15851690). Cytosolic in quiescent mast cells Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739)|
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1. J Biol Chem. 2000 Nov 10;275(45):35063-9. 2. J Virol. 2001 Jun;75(11):5043-8. 3. Biochem Biophys Res Commun. 2002 Feb 15;291(1):158-64. 4. Cell. 2004 Oct 15;119(2):195-208.
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