|Application ||IHC, E|
|Description||5'-nucleotidase, ecto (NT5E), also known as CD73 (Cluster of Differentiation 73). Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 126.96.36.199) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.|
|Immunogen||Purified recombinant fragment of NT5E expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||5'-nucleotidase, 5'-NT, 188.8.131.52, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE|
|Dilution||IHC~~1/200 - 1/1000|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NT5E Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
1. Oncol Rep. 2007 Jun;17(6):1341-6. 2. Neurochem Int. 2003 Dec;43(7):621-8.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.