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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NKX3A Antibody   

NKX3A Antibody

Purified Mouse Monoclonal Antibody

  • WB - NKX3A Antibody AO1380a
    Figure 1: Western blot analysis using NKX3A mouse mAb against LNCaP (1) cell lysate.
  • IHC - NKX3A Antibody AO1380a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human prostata tissues (A, B) using anti-NKX3A antibody with DAB staining.
  • IHC - NKX3A Antibody AO1380a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human Liver tissues using NKX3A mAb
  • FC - NKX3A Antibody AO1380a
    Figure 4: Flow cytometric analysis of PC-3 cells using anti-NKX3A mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99801
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4H4
Isotype IgG2b
Calculated MW 26.3kDa
Description Nkx3.1 is a transcription factor that may play an important role in regulating proliferation of glandular epithelium and in the formation of ducts in the prostate. It has been thought to be one of the target genes of the 8p21 loss of heterozygosity, common in prostate cancer. But neither disruption of the coding region of the gene, nor mutations have been found in prostate cancer.
Immunogen Purified recombinant fragment of human NKX3A expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 4824
Other Names Homeobox protein Nkx-3.1, Homeobox protein NK-3 homolog A, NKX3-1, NKX3.1, NKX3A
Dilution WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNKX3A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NKX3-1
Synonyms NKX3.1, NKX3A
Function Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108, ECO:0000269|PubMed:11137288}
Tissue Location Highly expressed in the prostate and, at a lower level, in the testis.
Research Areas
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1. Exp Mol Med. 2006 Dec 31;38(6):625-33. 2. Exp Biol Med (Maywood). 2008 Mar;233(3):297-309. 3.Mol Biol Rep. 2010 Mar;37(3):1505-12.

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$ 325.00
Cat# AO1380a
Availability: 7-10 days
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