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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EIF4B Antibody   

EIF4B Antibody

Purified Mouse Monoclonal Antibody

  • E - EIF4B Antibody AO1715a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - EIF4B Antibody AO1715a
    Figure 1: Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)
  • WB - EIF4B Antibody AO1715a
    Figure 2: Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.
  • FC - EIF4B Antibody AO1715a
    Figure 4: Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).
  • IF - EIF4B Antibody AO1715a
    Figure 5 : Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • IHC - EIF4B Antibody AO1715a
    Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P23588
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 1F5
Isotype IgG2b
Calculated MW 80kDa
Description The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA.It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F
Immunogen Purified recombinant fragment of human EIF4B expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1975
Other Names Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF4B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4B
Function Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Research Areas
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1. Mol Biol Cell. 2009 Jun;20(11):2673-83. 2. EMBO J. 2006 Jun 21;25(12):2781-91.

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$ 385.00
Cat# AO1715a
Availability: 7-10 days
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