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RAB4A Antibody

Purified Mouse Monoclonal Antibody

  • E - RAB4A Antibody AO1720a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - RAB4A Antibody AO1720a
    Figure 1: Western blot analysis using RAB4A mAb against human RAB4A (AA: 22-218) recombinant protein. (Expected MW is 47.9 kDa)
  • WB - RAB4A Antibody AO1720a
    Figure 2: Western blot analysis using RAB4A mouse mAb against Jurkat (1), HeLa (2), A549 (3), HEK293 (4), K562 (5), NIH3T3 (6), PC-12 (7), and COS7 (8) cell lysate.
  • IHC - RAB4A Antibody AO1720a
    Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RAB4A mouse mAb with DAB staining.
  • IHC - RAB4A Antibody AO1720a
    Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using RAB4A mouse mAb with DAB staining.
  • FC - RAB4A Antibody AO1720a
    Figure 5: Flow cytometric analysis of MCF-7 cells using RAB4A mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20338
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 4E11
Isotype IgG1
Calculated MW 23.8kDa
Description The protein encoded by this gene belongs to the rab family. Rab4A protein is involved in the regulation of procathepsin L secretion, it was either inhibited by expression of a Rab4A dominant-negative mutant or increased by overexpression of the wild-type Rab4A in human melanoma cells.
Immunogen Purified recombinant fragment of human RAB4A expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5867
Other Names Ras-related protein Rab-4A, RAB4A, RAB4
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB4A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB4A
Synonyms RAB4
Function Protein transport. Probably involved in vesicular traffic (By similarity).
Cellular Location Membrane; Peripheral membrane protein. Cytoplasm. Early endosome membrane {ECO:0000250|UniProtKB:P05714}; Peripheral membrane protein {ECO:0000250|UniProtKB:P05714}. Recycling endosome membrane {ECO:0000250|UniProtKB:P05714}; Peripheral membrane protein {ECO:0000250|UniProtKB:P05714}. Note=Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1
Research Areas
Citations (0)

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1. Biochem J. 2011 Jul 1;437(1):97-107. 2. Int J Cancer. 2011 Jan 1;128(1):192-205.

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$ 385.00
Cat# AO1720a
Availability: 7-10 days
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