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PAPLN Antibody

Purified Mouse Monoclonal Antibody

  • E - PAPLN Antibody AO1860a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PAPLN Antibody AO1860a
    Figure 1: Western blot analysis using PAPLN mAb against human PAPLN recombinant protein. (Expected MW is 36.4 kDa)
  • WB - PAPLN Antibody AO1860a
    Figure 2: Western blot analysis using PAPLN mAb against HEK293 (1) and PAPLN (AA: 766-870)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - PAPLN Antibody AO1860a
    Figure 3: Western blot analysis using PAPLN mouse mAb against Hela (1), HepG2 (2), OCM-1 (3), Raji (4), Jurkat (5), NIH/3T3 (6) cell lysate.
  • IF - PAPLN Antibody AO1860a
    Figure 4: Immunofluorescence analysis of NIH/3T3 cells using PAPLN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95428
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 5B2E5
Isotype IgG1
Calculated MW 137.7kDa
Description Papilin is an extracellular matrix glycoprotein involved in, thin matrix layers during gastrulation, matrix associated with wandering, phagocytic hemocytes, basement membranes and space-filling matrix during Drosophila development.Determination of its cDNA sequence led to the identification of Caenorhabditis and mammalian papilins. A distinctly conserved 'papilin cassette' of domains at the amino-end of papilins is also the carboxyl-end of the ADAMTS subgroup of secreted, matrix-associated metalloproteinases; this cassette contains one thrombospondin type 1 (TSR) domain, a specific cysteine-rich domain and several partial TSR domains. In vitro, papilin non-competitively inhibits procollagen N-proteinase, an ADAMTS metalloproteinase.
Immunogen Purified recombinant fragment of human PAPLN (AA: 766-870) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 89932
Other Names Papilin, PAPLN
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAPLN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Cellular Location Secreted.
Research Areas
Citations (0)

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This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. ; ;


1. Int J Biochem Cell Biol. 2004 Jun; 36(6):1079-84. 2. Development. 2000 Dec;127(24):5475-85.

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$ 385.00
Cat# AO1860a
Availability: 7-10 days
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