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WTAP Antibody

Purified Mouse Monoclonal Antibody

  • E - WTAP Antibody AO2021a

    Figure 1:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - WTAP Antibody AO2021a

    Figure 2:Western blot analysis using WTAP mAb against human WTAP (AA: 91-201) recombinant protein. (Expected MW is 38.7 kDa)

  • WB - WTAP Antibody AO2021a

    Figure 3:Western blot analysis using WTAP mAb against HEK293 (1) and WTAP (AA: 91-201)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - WTAP Antibody AO2021a

    Figure 4:Flow cytometric analysis of Hela cells using WTAP mouse mAb (green) and negative control (red).

  • IHC - WTAP Antibody AO2021a

    Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using WTAP mouse mAb with DAB staining.

  • IHC - WTAP Antibody AO2021a

    Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using WTAP mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15007
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6B6B8
Isotype IgG1
Calculated MW 44.2kDa
Description The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms.
Immunogen Purified recombinant fragment of human WTAP (AA: 91-201) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 9589
Other Names Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, hFL(2)D, WT1-associated protein, Wilms tumor 1-associating protein, WTAP, KIAA0105
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWTAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WTAP {ECO:0000303|PubMed:11001926, ECO:0000312|HGNC:HGNC:16846}
Function Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).
Cellular Location Nucleus speckle. Nucleus, nucleoplasm. Cytoplasm {ECO:0000250|UniProtKB:Q9ER69}. Note=Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus {ECO:0000250|UniProtKB:Q9ER69}
Tissue Location Ubiquitously expressed.
Citations (0)

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J Gastroenterol. 2013 Nov;48(11):1271-82.Cancer Sci. 2012 Dec;103(12):2102-9.

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$ 385.00
Cat# AO2021a
Availability: 7-10 days
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