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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RALA Antibody   

RALA Antibody

Purified Mouse Monoclonal Antibody

  • E - RALA Antibody AO2092a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - RALA Antibody AO2092a

    Figure 2:Western blot analysis using RALA mAb against human RALA (AA: 71-203) recombinant protein. (Expected MW is 41.5 kDa)

  • WB - RALA Antibody AO2092a

    Figure 3:Western blot analysis using RALA mAb against HEK293 (1) and RALA (AA: 71-203)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - RALA Antibody AO2092a

    Figure 4:Western blot analysis using RALA mouse mAb against HepG2 (1), MCF-7 (2), A549 (3), K562 (4), Raji (5), MOLT4 (6), Hela (7), COS7 (8), and NIH3T3 (9) cell lysate.

  • IF - RALA Antibody AO2092a

    Figure 5:Immunofluorescence analysis of Hela cells using RALA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - RALA Antibody AO2092a

    Figure 6:Flow cytometric analysis of MCF-7 cells using RALA mouse mAb (green) and negative control (red).

  • IHC - RALA Antibody AO2092a

    Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using RALA mouse mAb with DAB staining.

  • IHC - RALA Antibody AO2092a

    Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RALA mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11233
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 4G8C7
Isotype IgG1
Calculated MW 23.6kDa
Description The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.
Immunogen Purified recombinant fragment of human RALA (AA: 71-203) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5898
Other Names Ras-related protein Ral-A, RALA, RAL
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRALA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms RAL
Function Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed:20005108). Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage- independent proliferation of transformed cells (PubMed:19306925). During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody (PubMed:18756269).
Cellular Location Cell membrane; Lipid- anchor; Cytoplasmic side. Cleavage furrow. Midbody, Midbody ring. Note=Predominantly at the cell surface in the absence of LPA. In the presence of LPA, colocalizes with LPAR1 and LPAR2 in endocytic vesicles (PubMed:19306925). May colocalize with CNTRL/centriolin at the midbody ring (PubMed:16213214). However, localization at the midbody at late cytokinesis was not confirmed (PubMed:18756269)
Research Areas
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1.Cell Signal. 2012 Jun;24(6):1134-40.2.Int J Immunopathol Pharmacol. 2009 Jul-Sep;22(3):735-43.

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$ 385.00
Cat# AO2092a
Availability: 7-10 days
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