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RAD18 Antibody

Purified Mouse Monoclonal Antibody

  • E - RAD18 Antibody AO2118a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - RAD18 Antibody AO2118a

    Figure 2:Western blot analysis using RAD18 mAb against human RAD18 (AA: 332-430) recombinant protein. (Expected MW is 37.3 kDa)

  • WB - RAD18 Antibody AO2118a

    Figure 3:Western blot analysis using RAD18 mAb against HEK293 (1) and RAD18 (AA: 332-430)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - RAD18 Antibody AO2118a

    Figure 4:Flow cytometric analysis of K562 cells using RAD18 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NS91
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2G8D12
Isotype IgG1
Calculated MW 56.2kDa
Description The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Immunogen Purified recombinant fragment of human RAD18 (AA: 332-430) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 56852
Other Names E3 ubiquitin-protein ligase RAD18, 6.3.2.-, Postreplication repair protein RAD18, hHR18, hRAD18, RING finger protein 73, RAD18, RNF73
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAD18 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAD18
Synonyms RNF73
Function E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono- ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
Cellular Location Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with chromatin (PubMed:25931565). Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077). Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607). Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565)
Research Areas
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1.Nucleic Acids Res. 2013 Feb 1;41(4):2296-312.2.J Exp Clin Cancer Res. 2009 Jul 25;28:106.

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$ 385.00
Cat# AO2118a
Availability: 7-10 days
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