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phospho-NLRC4(Ser-533) Antibody

Purified Rat Monoclonal Antibody

  • E - phospho-NLRC4(Ser-533) Antibody AO2202a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • IF - phospho-NLRC4(Ser-533) Antibody AO2202a

    Figure 2:Immunofluorescence analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • IF - phospho-NLRC4(Ser-533) Antibody AO2202a

    Figure 3:Immunofluorescence analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - phospho-NLRC4(Ser-533) Antibody AO2202a

    Figure 4:Flow cytometric analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb (green) and negative control (red).

  • FC - phospho-NLRC4(Ser-533) Antibody AO2202a

    Figure 5:Flow cytometric analysis of HepG2 cells using phospho-NLRC4(Ser-533) rat mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q3UP24
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 9A6A1
Isotype IgG1
Calculated MW 116.7kDa
Description NLRC4 is a cytosolic NOD (nucleotide binding and oligomerization domain)-like receptor (NLR) that can trigger inflammasome formation in response to bacterial flagellin, an immunodominant antigen in the intestine.
Immunogen Synthesized peptide of mouse phospho-NLRC4(Ser-533) (AA: 525-538) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 268973
Other Names NLR family CARD domain-containing protein 4, Caspase recruitment domain-containing protein 12, Ice protease-activating factor, Ipaf, Nlrc4, Card12, Ipaf
Dilution E~~1/10000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsphospho-NLRC4(Ser-533) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Nlrc4
Synonyms Card12, Ipaf
Function Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. It senses pathogenic proteins of the type III secretion system (T3SS) and type IV secretion system (T4SS) such as flagellin and PrgJ-like rod proteins via the Naip proteins (Naip1, Naip2 or Naip5): specific Naip proteins recognize and bind pathogenic proteins, driving assembly and activation of the NLRC4 inflammasome. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri and fungal pathogen C.albicans. In intestine, the NLRC4 inflammasome is able to discriminate between commensal and pathogenic bacteria and specifically drives production of interleukin-1 beta (IL1B) in response to infection by Salmonella or P.aeruginosa. In case of L.pneumophila infection the inflammasome acts by activating caspase-7.
Cellular Location Cytoplasm, cytosol
Tissue Location Expressed by intestinal mononuclear phagocytes.
Research Areas
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1.Mucosal Immunol. 2014 Jul;7(4):775-85. 2.Mucosal Immunol. 2012 May;5(3):288-98.

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$ 385.00
Cat# AO2202a
Availability: 7-10 days
Bulk Size
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