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GFPT1 Antibody

Purified Mouse Monoclonal Antibody

  • E - GFPT1 Antibody AO2212a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - GFPT1 Antibody AO2212a

    Figure 2:Western blot analysis using GFPT1 mAb against human GFPT1 (AA: 536-681) recombinant protein. (Expected MW is 42.3 kDa)

  • WB - GFPT1 Antibody AO2212a

    Figure 3:Western blot analysis using GFPT1 mAb against HEK293 (1) and GFPT1 (AA: 536-681)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - GFPT1 Antibody AO2212a

    Figure 4:Western blot analysis using GFPT1 mouse mAb against Hela (1), HepG2 (2), HEK293 (3), BEL-7402 (4), SMMC-7721 (5), SK-MES-1 (6), C6 (7), and COS7 (8) cell lysate.

  • IF - GFPT1 Antibody AO2212a

    Figure 5:Immunofluorescence analysis of Hela cells using GFPT1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - GFPT1 Antibody AO2212a

    Figure 6:Flow cytometric analysis of Hela cells using GFPT1 mouse mAb (green) and negative control (red).

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q06210
Reactivity Human, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 1F1B9
Isotype IgG1
Calculated MW 78.8kDa
Description This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.
Immunogen Purified recombinant fragment of human GFPT1 (AA: 536-681) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2673
Other Names Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1,, D-fructose-6-phosphate amidotransferase 1, Glutamine:fructose-6-phosphate amidotransferase 1, GFAT 1, GFAT1, Hexosephosphate aminotransferase 1, GFPT1, GFAT, GFPT
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGFPT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GFPT1
Synonyms GFAT, GFPT
Function Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.
Tissue Location Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle.
Research Areas
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1.Neurology. 2013 Jul 23;81(4):370-8.2.Hum Mol Genet. 2013 Jul 15;22(14):2905-13.

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$ 385.00
Cat# AO2212a
Availability: 7-10 days
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