|Application ||WB, FC, E|
|Description||This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.|
|Immunogen||Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, eRF1, Protein Cl1, TB3-1, ETF1, ERF1, RF1, SUP45L1|
WB~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ERF1, RF1, SUP45L1|
|Function||Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA (PubMed:7990965, PubMed:24486019). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.|
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1.Nucleic Acids Res. 2013 Apr;41(8):4573-86. 2.Protein Sci. 2012 Jun;21(6):896-903.
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