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RF1 Antibody

Purified Mouse Monoclonal Antibody

  • E - RF1 Antibody AO2222a

    Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • WB - RF1 Antibody AO2222a

    Figure 2:Western blot analysis using RF1 mAb against human RF1 (AA: 288-437) recombinant protein. (Expected MW is 43 kDa)

  • WB - RF1 Antibody AO2222a

    Figure 3:Western blot analysis using RF1 mAb against HEK293 (1) and RF1 (AA: 288-437)-hIgGFc transfected HEK293 (2) cell lysate.

  • FC - RF1 Antibody AO2222a

    Figure 5:Flow cytometric analysis of Hela cells using RF1 mouse mAb (green) and negative control (red).

  • FC - RF1 Antibody AO2222a

    Figure 6:Flow cytometric analysis of HepG2 cells using RF1 mouse mAb (green) and negative control (red).

  • WB - RF1 Antibody AO2222a

    Figure 6:Western blot analysis using *** mouse mAb against MCF-7 (1), T47D (2), MOLT4 (3), and Raji (4) cell lysate.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P62495
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4F9H12
Isotype IgG1
Calculated MW 49kDa
Description This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
Immunogen Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2107
Other Names Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, eRF1, Protein Cl1, TB3-1, ETF1, ERF1, RF1, SUP45L1
Dilution E~~1/10000
WB~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ETF1
Synonyms ERF1, RF1, SUP45L1
Function Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA (PubMed:7990965, PubMed:24486019). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
Cellular Location Cytoplasm.
Citations (0)

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1.Nucleic Acids Res. 2013 Apr;41(8):4573-86. 2.Protein Sci. 2012 Jun;21(6):896-903.

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$ 385.00
Cat# AO2222a
Availability: 7-10 days
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