|Application ||WB, FC, E|
|Description||This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). ;|
|Immunogen||Purified recombinant fragment of human MIB1 (AA: 6-221) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Application Note||ELISA: 1/10000|
WB: 1/500 - 1/2000
FCM: 1/200 - 1/400
|Other Names||MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse Monoclonal Antibody to MIB1 is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DIP1, KIAA1323, ZZANK2|
|Function||E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite Cell membrane. Note=Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear Displaced from centriolar satellites in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock|
|Tissue Location||Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.|
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1.J Cell Sci. 2015 May 1;128(9):1674-82. ; 2.Cell Res. 2012 Mar;22(3):603-6. ;
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