|Application ||WB, IHC-P, IF, FC, E|
|Other Accession||Q3SYZ4, NP_001340.2|
|Calculated MW||57136 Da|
|Antigen Region||154-183 aa|
|Other Names||Aspartate--tRNA ligase, cytoplasmic, Aspartyl-tRNA synthetase, AspRS, Cell proliferation-inducing gene 40 protein, DARS|
|Target/Specificity||This DARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the N-terminal region of human DARS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DARS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.|
|Cellular Location||Cytoplasm, cytosol|
|Tissue Location||Expression in the developing and adult brain shows similar patterns. Highly expressed in the ventricular and subventricular zones, including hippocampal subfields, the midlateral temporaal cortex and the frontal polar cortex. The cerebellum, cereral cortex, hippocampus, and lateral ventricle show preferential neuronal expression. Expression in the peripheral neurons is evident in the colon|
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Provided below are standard protocols that you may find useful for product applications.
Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Tu, L.C., et al. Mol. Cell Proteomics 6(4):575-588(2007)
Ling, C., et al. J. Biol. Chem. 280(41):34755-34763(2005)
Bonnefond, L., et al. Biochemistry 44(12):4805-4816(2005)
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