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SOX15 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - SOX15 Antibody (Center) AP12473c
    Anti-SOX15 Antibody (Center) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SOX15 Antibody (Center) AP12473c
    SOX15 Antibody (Center) (Cat. #AP12473c) western blot analysis in human placenta and mouse brain tissue lysates (35ug/lane).This demonstrates the SOX15 antibody detected the SOX15 protein (arrow).
  • IHC-P - SOX15 Antibody (Center) AP12473c
    SOX15 Antibody (Center) (Cat. #AP12473c)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SOX15 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60248
Other Accession NP_008873.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 96-125 aa
Additional Information
Other Names Protein SOX-15, Protein SOX-12, Protein SOX-20, SOX15, SOX12, SOX20, SOX26, SOX27
Target/Specificity This SOX15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the Central region of human SOX15.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSOX15 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOX15
Synonyms SOX12, SOX20, SOX26, SOX27
Function Binds to the 5'-AACAAT-3' sequence.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00267}
Tissue Location Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis. EMBL; AB006867; BAA25663.1; -; mRNA EMBL; AK301687; BAG63159.1; -; mRNA EMBL; AC016876; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471108; EAW90156.1; -; Genomic_DNA EMBL; CH471108; EAW90157.1; -; Genomic_DNA EMBL; BC000985; AAH00985.1; -; mRNA EMBL; BC072003; AAH72003.1; -; mRNA EMBL; X73039; CAA51520.1; -; Genomic_DNA EMBL; AJ006222; CAA06920.1; -; mRNA EMBL; AB025355; BAA78784.1; -; Genomic_DNA CCDS; CCDS32549.1; -. [O60248-1] PIR; S34119; S34119 RefSeq; NP_008873.1; NM_006942.1. [O60248-1] UniGene; Hs.95582; - ProteinModelPortal; O60248; - SMR; O60248; - BioGrid; 112548; 3 IntAct; O60248; 2 STRING; 9606.ENSP00000355354; - iPTMnet; O60248; - PhosphoSitePlus; O60248; - BioMuta; SOX15; - EPD; O60248; - MaxQB; O60248; - PaxDb; O60248; - PeptideAtlas; O60248; - PRIDE; O60248; - ProteomicsDB; 49282; - DNASU; 6665; - Ensembl; ENST00000250055; ENSP00000355354; ENSG00000129194. [O60248-1] Ensembl; ENST00000538513; ENSP00000439311; ENSG00000129194. [O60248-1] Ensembl; ENST00000570788; ENSP00000458286; ENSG00000129194. [O60248-2] GeneID; 6665; - KEGG; hsa:6665; - UCSC; uc002ghy.2; human. [O60248-1] CTD; 6665; - DisGeNET; 6665; - EuPathDB; HostDB:ENSG00000129194.7; - GeneCards; SOX15; - HGNC; HGNC:11196; SOX15 HPA; HPA067196; - HPA; HPA074049; - MIM; 601297; gene neXtProt; NX_O60248; - OpenTargets; ENSG00000129194; - PharmGKB; PA36033; - eggNOG; KOG0527; Eukaryota eggNOG; ENOG410XT0K; LUCA GeneTree; ENSGT00760000118988; - HOGENOM; HOG000231647; - HOVERGEN; HBG055512; - InParanoid; O60248; - KO; K09271; - OMA; YGSSHCK; - OrthoDB; EOG091G0M3O; - PhylomeDB; O60248; - TreeFam; TF351735; - GeneWiki; SOX15; - GenomeRNAi; 6665; - PRO; PR:O60248; - Proteomes; UP000005640; Chromosome 17 Bgee; ENSG00000129194; - CleanEx; HS_SOX12; - CleanEx; HS_SOX15; - Genevisible; O60248; HS GO; GO:0005737; C:cytoplasm; IEA:Ensembl GO; GO:0044798; C:nuclear transcription factor complex; IEA:Ensembl GO; GO:0005634; C:nucleus; NAS:UniProtKB GO; GO:0003682; F:chromatin binding; IEA:Ensembl GO; GO:0003677; F:DNA binding; NAS:UniProtKB GO; GO:0003700; F:DNA binding transcription factor activity; NAS:UniProtKB GO; GO:0046982; F:protein heterodimerization activity; IEA:Ensembl GO; GO:0000981; F:RNA polymerase II transcription factor activity, sequence-specific DNA binding; ISA:NTNU_SB GO; GO:0030154; P:cell differentiation; ISS:BHF-UCL GO; GO:0006325; P:chromatin organization; NAS:UniProtKB GO; GO:0008584; P:male gonad development; TAS:ProtInc GO; GO:0048627; P:myoblast development; ISS:BHF-UCL GO; GO:0045843; P:negative regulation of striated muscle tissue development; ISS:BHF-UCL GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:BHF-UCL GO; GO:0070318; P:positive regulation of G0 to G1 transition; ISS:BHF-UCL GO; GO:2000288; P:positive regulation of myoblast proliferation; ISS:BHF-UCL GO; GO:0014718; P:positive regulation of satellite cell activation involved in skeletal muscle regeneration; ISS:BHF-UCL GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:BHF-UCL GO; GO:0006357; P:regulation of transcription by RNA polymerase II; TAS:ProtInc GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB GO; GO:0043403; P:skeletal muscle tissue regeneration; IEA:Ensembl Gene3D;; -; 1 InterPro; IPR009071; HMG_box_dom InterPro; IPR036910; HMG_box_dom_sf InterPro; IPR031269; SOX15 PANTHER; PTHR10270:SF45; PTHR10270:SF45; 1 Pfam; PF00505; HMG_box; 1 SMART; SM00398; HMG; 1 SUPFAM; SSF47095; SSF47095; 1 PROSITE; PS50118; HMG_BOX_2; 1 1: Evidence at protein level; Alternative splicing; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome CHAIN 1 233 Protein SOX-15 /FTId=PRO_0000048762 DNA_BIND 49 117 HMG box. {ECO:0000255|PROSITE- ProRule:PRU00267} MOD_RES 37 37 Phosphoserine VAR_SEQ 179 233 Missing (in isoform 2) /FTId=VSP_056668 CONFLICT 25 27 SSS -> AAA (in Ref. 6). CONFLICT 69 70 QQ -> HE (in Ref. 7; CAA51520) CONFLICT 191 191 S -> F (in Ref. 9; BAA78784) CONFLICT 216 218 PTP -> STS (in Ref. 9; BAA78784) CONFLICT 225 226 GA -> VP (in Ref. 9; BAA78784) SEQUENCE 233 AA; 25251 MW; 6B52AE455C7C8CCE CRC64; MALPGSSQDQ AWSLEPPAAT AAASSSSGPQ EREGAGSPAA PGTLPLEKVK RPMNAFMVWS SAQRRQMAQQ NPKMHNSEIS KRLGAQWKLL DEDEKRPFVE EAKRLRARHL RDYPDYKYRP RRKAKSSGAG PSRCGQGRGN LASGGPLWGP GYATTQPSRG FGYRPPSYST AYLPGSYGSS HCKLEAPSPC SLPQSDPRLQ GELLPTYTHY LPPGSPTPYN PPLAGAPMPL THL
Research Areas
Citations (0)

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This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. [provided by RefSeq].


Sakai, N., et al. J. Endocrinol. 198(3):489-497(2008)
Yan, H.T., et al. Mol. Cells 24(3):323-328(2007)
Schepers, G.E., et al. Dev. Cell 3(2):167-170(2002)
Wilson, M., et al. Curr. Opin. Genet. Dev. 12(4):441-446(2002)
Miyashita, A., et al. Gene 237(1):53-60(1999)

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Cat# AP12473c
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