|Application ||FC, WB, IHC-P, E|
|Other Accession||Q14184, P70611, Q7TNF0, NP_003577.2|
|Calculated MW||43959 Da|
|Antigen Region||277-306 aa|
|Other Names||Double C2-like domain-containing protein alpha, Doc2, Doc2-alpha, DOC2A|
|Target/Specificity||This DOC2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the Central region of human DOC2A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DOC2A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.|
|Cellular Location||Lysosome. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Note=Colocalizes to synaptic vesicles|
|Tissue Location||Predominantly expressed in brain. Also expressed in testis.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release.
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Glessner, J.T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(23):10584-10589(2010)
Guilmatre, A., et al. Arch. Gen. Psychiatry 66(9):947-956(2009)
Kumar, R.A., et al. PLoS ONE 4 (2), E4582 (2009) :
Higashio, H., et al. J. Immunol. 180(7):4774-4784(2008)
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