Bak Antibody (BH3 Domain Specific)
Purified Rabbit Polyclonal Antibody (Pab)
- CITATIONS: 1
|Application ||IHC-P, WB, E|
|Calculated MW||23409 Da|
|Antigen Region||56-91 aa|
|Other Names||Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1|
|Target/Specificity||This Bak antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-91 amino acids from human Bak.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Bak Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BAK, BCL2L7, CDN1|
|Function||Plays a role in the mitochondrial apoptosic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.|
|Cellular Location||Mitochondrion outer membrane; Single-pass membrane protein|
|Tissue Location||Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle|
Provided below are standard protocols that you may find useful for product applications.
BAK belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAK localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
Cartron, P.F., et al., Mol. Cell. Biol. 23(13):4701-4712 (2003).
Mikhailov, V., et al., J. Biol. Chem. 278(7):5367-5376 (2003).
Werner, A.B., et al., J. Biol. Chem. 277(25):22781-22788 (2002).
Bellosillo, B., et al., Blood 100(5):1810-1816 (2002).
Grutkoski, P.S., et al., Shock 17(1):47-54 (2002).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.