|Application ||WB, IHC-P, E|
|Calculated MW||62343 Da|
|Antigen Region||364-393 aa|
|Other Names||Wee1-like protein kinase 2, Wee1-like protein kinase 1B, Wee1B kinase, mWee1B, Wee2, Wee1b|
|Target/Specificity||This Mouse Wee2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from the Central region of mouse Wee2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse Wee2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.|
|Cellular Location||Cytoplasm. Nucleus. Note=Localizes mainly in the nucleus. Exported from the nucleus to the cytoplasm before germinal vesicle breakdown (GVBD), allowing meiosis resumption|
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Provided below are standard protocols that you may find useful for product applications.
Phosphorylates and inhibits CDK1. May act as a negative regulator of entry into mitosis (G2 to M transition).
Oh, J.S., et al. J. Cell Biol. 188(2):199-207(2010)
Joshi, S., et al. BMC Dev. Biol. 7, 67 (2007) :
Han, S.J., et al. Curr. Biol. 15(18):1670-1676(2005)
Nakanishi, M., et al. Genes Cells 5(10):839-847(2000)
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