|Application ||WB, E|
|Calculated MW||72996 Da|
|Antigen Region||371-400 aa|
|Other Names||E3 ubiquitin-protein ligase AMFR, 632-, Autocrine motility factor receptor, AMF receptor, RING finger protein 45, gp78, AMFR, RNF45|
|Target/Specificity||This AMFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371-400 amino acids from the Central region of human AMFR.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AMFR Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.|
|Cellular Location||Endoplasmic reticulum membrane; Multi- pass membrane protein Note=Palmitoylation promotes localization to the peripheral endoplasmic reticulum.|
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Provided below are standard protocols that you may find useful for product applications.
Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells.
Joshi, B., et al. J. Biol. Chem. 285(12):8830-8839(2010)
Bernardi, K.M., et al. Mol. Biol. Cell 21(1):140-151(2010)
Ballar, P., et al. Int. J. Biochem. Cell Biol. 42(1):167-173(2010)
Shmueli, A., et al. Biochem. Biophys. Res. Commun. 390(3):758-762(2009)
Ying, Z., et al. Hum. Mol. Genet. 18(22):4268-4281(2009)
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