|Application ||WB, E|
|Calculated MW||48805 Da|
|Antigen Region||384-412 aa|
|Other Names||Diphthamide biosynthesis protein 1, DPH1 homolog, HsDph1, Diphthamide biosynthesis protein 2 homolog-like 1, DPH-like 1, DPH2-like 1, Diphthamide biosynthesis protein 2-like, Ovarian cancer-associated gene 1 protein, DPH1, DPH2L, DPH2L1, OVCA1|
|Target/Specificity||This DPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-412 amino acids from the C-terminal region of human DPH1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DPH1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DPH2L, DPH2L1, OVCA1|
|Function||Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers (By similarity). Plays a role in embryonic growth, organogenesis and postnatal survival (By similarity).|
|Cellular Location||Nucleus. Cytoplasm. Note=Punctate, primarily perinuclear localization|
|Tissue Location||Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, mammary gland, colon, small intestine, testis and ovary. Reduced expression in primary breast and ovarian tumors|
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Provided below are standard protocols that you may find useful for product applications.
Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM].
Liu, S., et al. Mol. Cell. Biol. 24(21):9487-9497(2004)
Cardoso, C., et al. Am. J. Hum. Genet. 72(4):918-930(2003)
Chen, C.M., et al. Biochem. Biophys. Res. Commun. 286(5):1019-1026(2001)
Salicioni, A.M., et al. Genomics 69(1):54-62(2000)
Bruening, W., et al. Cancer Res. 59(19):4973-4983(1999)
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