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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Casc5 Antibody (C-term)   

Mouse Casc5 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Mouse Casc5 Antibody (C-term) AP19227b
    Mouse Casc5 Antibody (C-term) (Cat. #AP19227b) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Casc5 antibody detected the Casc5 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q66JQ7
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 1557-1583 aa
Additional Information
Other Names Protein CASC5, Cancer susceptibility candidate gene 5 protein homolog, Kinetochore-null protein 1, Casc5, Knl1
Target/Specificity This Mouse Casc5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1557-1583 amino acids from the C-terminal region of mouse Casc5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Casc5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Knl1 {ECO:0000312|MGI:MGI:1923714}
Synonyms Casc5
Function Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Directly links spindle checkpoint proteins BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q8NG31}. Chromosome, centromere, kinetochore {ECO:0000250|UniProtKB:Q8NG31}. Note=Weakly expressed in interphase nuclei. Expression increases from prophase to late anaphase, but greatly diminishes from the telophase and cytokinesis to early G1 phase of cell cycle (By similarity) {ECO:0000250|UniProtKB:Q8NG31} EMBL; AL772264; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC080815; AAH80815.1; -; mRNA EMBL; AK009772; BAB26494.1; -; mRNA EMBL; AK033132; BAC28167.2; -; mRNA UniGene; Mm.202518; - UniGene; Mm.392877; - ProteinModelPortal; Q66JQ7; - SMR; Q66JQ7; - IntAct; Q66JQ7; 15 MINT; Q66JQ7; - STRING; 10090.ENSMUSP00000028802; - iPTMnet; Q66JQ7; - PhosphoSitePlus; Q66JQ7; - EPD; Q66JQ7; - MaxQB; Q66JQ7; - PaxDb; Q66JQ7; - PRIDE; Q66JQ7; - Ensembl; ENSMUST00000028799; ENSMUSP00000028799; ENSMUSG00000027326 UCSC; uc008ltb.1; mouse MGI; MGI:1923714; Knl1 eggNOG; ENOG410IF9C; Eukaryota eggNOG; ENOG410XZXJ; LUCA GeneTree; ENSGT00410000025918; - HOGENOM; HOG000246954; - HOVERGEN; HBG080986; - InParanoid; Q66JQ7; - Reactome; R-MMU-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal Reactome; R-MMU-2467813; Separation of Sister Chromatids Reactome; R-MMU-2500257; Resolution of Sister Chromatid Cohesion Reactome; R-MMU-5663220; RHO GTPases Activate Formins Reactome; R-MMU-606279; Deposition of new CENPA-containing nucleosomes at the centromere Reactome; R-MMU-68877; Mitotic Prometaphase PRO; PR:Q66JQ7; - Proteomes; UP000000589; Chromosome 2 Bgee; ENSMUSG00000027326; - CleanEx; MM_CASC5; - ExpressionAtlas; Q66JQ7; baseline and differential Genevisible; Q66JQ7; MM GO; GO:0001669; C:acrosomal vesicle; ISO:MGI GO; GO:0000777; C:condensed chromosome kinetochore; ISO:MGI GO; GO:0005829; C:cytosol; ISO:MGI GO; GO:0016604; C:nuclear body; ISO:MGI GO; GO:0005654; C:nucleoplasm; ISO:MGI GO; GO:0005634; C:nucleus; ISO:MGI GO; GO:0008608; P:attachment of spindle microtubules to kinetochore; ISO:MGI GO; GO:0051301; P:cell division; IEA:UniProtKB-KW GO; GO:0010923; P:negative regulation of phosphatase activity; ISS:UniProtKB GO; GO:0034501; P:protein localization to kinetochore; ISO:MGI InterPro; IPR037388; Blinkin PANTHER; PTHR16520; PTHR16520; 1 1: Evidence at protein level; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Complete proteome; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Reference proteome; Repeat CHAIN 1 1612 Kinetochore scaffold 1 /FTId=PRO_0000235983 REPEAT 723 827 1 REPEAT 923 1027 2 REGION 723 1027 2 X 104 AA approximate repeats MOTIF 1577 1590 Nuclear localization signal MOD_RES 32 32 Phosphoserine {ECO:0000250|UniProtKB:Q8NG31} MOD_RES 60 60 Phosphoserine {ECO:0000250|UniProtKB:Q8NG31} MOD_RES 538 538 Phosphoserine {ECO:0000250|UniProtKB:Q8NG31} MOD_RES 540 540 Phosphothreonine {ECO:0000250|UniProtKB:Q8NG31} MOD_RES 794 794 Phosphoserine MOD_RES 878 878 Phosphoserine {ECO:0000250|UniProtKB:Q8NG31} MOD_RES 1243 1243 Phosphoserine {ECO:0000250|UniProtKB:Q8NG31} MOD_RES 1464 1464 Phosphoserine {ECO:0000250|UniProtKB:Q8NG31} CONFLICT 128 128 D -> G (in Ref. 2; AAH80815) CONFLICT 375 375 H -> R (in Ref. 2; AAH80815) CONFLICT 1151 1151 P -> L (in Ref. 2; AAH80815) SEQUENCE 1612 AA; 179337 MW; A9BE2BB20E008621 CRC64; MDGVYSEANE ENDNTQRPVR RQHSSILKPP RSPLQDLKCG NQTNQEPNPP RKRKSSRRVS FADTIKVFQT ESHMKTERNS EISGMNTLLC APIQTQMQQK EFSITDCNHE RKHANDQTVI FSDENQMDLT ASHTVMITKG LSDCTKNENS TKIDTTSFLE NLKHHAANSR IKKDLACSTV SLSQNIFSEK INSDNFIKRL KTGKHISSST ELDKENAEIP VYSKDSNSAS STYQMHASLG VDENSSNRTR IFREQDDGMN LTQCHTACIK TWIPPSTEAK IGEFKGDKTI YGNECMELTT NYTIQVLSSE NNLSERETQT QNGMNVTTVD GATAPAPEKK TALKDKLNAA FQGSFPNPEN KIHIIKCHPI ESETHTVTQI SSQSASTLAV TSKSICSSPA IEGYKTIFHS SSNDAMELTK CLSAMEEEKK LLKADDKYSK ICTNPDAGPL REKTIYLEED SMDITKSHTV AIDNKIFKHD QENIKKEIIA IPIFEKEMVL RNLMPMSKDE KRDVNYISVP QVSKESLQRS QTNTLSVSLT DKKMEFLADE DMDLTKSHTT KLSQVIPTTF DLASKNVTKS YSHSKSPLNE WESLDKQVVL GQHSKLPLPQ RKDRDDPDCS HHKIMYSEEL QTMDLTKSHT IVIGFGPSEV QEHSKINLEH KNSQLTAESI QTAVNVPAAN SRVVTTNDMD MLKDRSTHKP ELLKEKQNIK IYGRKSIGRL KIDKTILFSE GNEGDMDITK SCTVKINHRS LLDKHDSHLV SLAGTSKTIL HARGQVEMEI NRSHTTPLEC KIISPSDITP GDLDKTMMSI DDHEELDMTK SHTVFIDYQA EAKGVLPDRL DFQLSKKESL QKPKVTSLAE EIYISKNSES NHLPAKGSQL TILEEGSNSG LGEETNDAQK PGFLNELLSG KTQRRKSLSL KNKSITFPEN DKSYREIPQS SAVEINNETR LEDRKGFSFV PLAGTSKPVL SAYGPEDMEI SIGQTTASEY KTVPPEEITT IPMDKTVMFV DNFGDLDVTR SHTVFIDCQA KEKVLDEYTN LGIQKTKTLS GSEGDTHIQE ITKNPAAQHK HHMTTVIPSS TVVSDQSSMK IKFHKADRDE EVKGKEVEAN MLKQTKPESC LLNITDGKNV DFTSSYTADV CRSSDKYSSL PNISSSDNSG GNTMSLCDKN KEKAYNCQVP NEFTYAAILP STYHMDSKKL SVFPPCPSKE VTQTESAIAL LKDEDPVEEP LGEMATFNSK HVSLNLAKDQ TEAFVDVSVA SQPHLSAQQS PSTQKGQDVA RRDEGILAKA GKKALPFLLE NVAASTWENE SKIPTNVEHF AVTYEKELSI SIQTDKCNTN VQSPSNSALT TQVIQTHANA EGALDFLVPS TVSCFSSTKP SLSNLNRKTE EVLDFQTVNL LPPAEQLLEE GSQAHSMSIV QATEIYRLGS RNDRDEESKT FCNEAETTSV PLKTAVKDKT RRCSLGIFLP KLPSKRSCSI TGVDDLEQIL ADAADLTQLE TQPVCSKDPG IGSVAAKLNL SPSQFINEEN LPVYPGEILS SDSVSLDIEE SVLIDTSQRE SLPSENKTEN CRAQKRTRVE ENDVTNEKKI RTHDSAQDQE VSCLEPKNVL EF
Research Areas
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Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Directly links spindle checkpoint proteins BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore (By similarity).

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$ 325.00
$ 109.00
Cat# AP19227b
Availability: In Stock
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