|Application ||WB, E|
|Other Accession||Q6ZPY2, NP_056142.2|
|Calculated MW||113928 Da|
|Antigen Region||912-941 aa|
|Other Names||Protein SMG5, EST1-like protein B, LPTS-RP1, LPTS-interacting protein, SMG-5 homolog, hSMG-5, SMG5, EST1B, KIAA1089|
|Target/Specificity||This SMG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 912-941 amino acids from the C-terminal region of human SMG5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SMG5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity.|
|Cellular Location||Cytoplasm. Nucleus. Note=Predominantly cytoplasmic, and nuclear. Shuttles between nucleus and cytoplasm Detected in cytoplasmic mRNA decay bodies|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al., 2003 [PubMed 14636577]).
Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010)
Glavan, F., et al. EMBO J. 25(21):5117-5125(2006)
Lee, H., et al. Mol. Cell. Biol. 26(14):5259-5269(2006)
Azzalin, C.M., et al. Curr. Biol. 16(4):433-439(2006)
Unterholzner, L., et al. Mol. Cell 16(4):587-596(2004)
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