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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ACO2 Antibody (Center)   

ACO2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ACO2 Antibody (Center) AP1936C
    Anti-Aconitase Antibody at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ACO2 Antibody (Center) AP1936C
    Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (Center) (Cat# AP1936c). Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ?. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.
  • WB - ACO2 Antibody (Center) AP1936C
    ACO2 (Cat. #AP1936c) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Aconitase antibody detected the Aconitase protein (arrow).
  • IHC-P - ACO2 Antibody (Center) AP1936C
    Formalin-fixed and paraffin-embedded human Heart tissue reacted with ACO2 Antibody (Center)(Cat.#AP1936c), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99798
Other Accession Q9ER34, P16276, Q99KI0, P20004
Reactivity Human, Rat
Predicted Bovine, Mouse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 85425 Da
Antigen Region 438-467 aa
Additional Information
Gene ID 50
Other Names Aconitate hydratase, mitochondrial, Aconitase, Citrate hydro-lyase, ACO2
Target/Specificity This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the Central region of human ACO2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACO2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACO2
Function Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Cellular Location Mitochondrion {ECO:0000250|UniProtKB:P16276}
Research Areas
Citations ( 0 )


ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.


Juang, H.H., Mol. Genet. Metab. 81(3):244-252 (2004).
Bota, D.A., et al., Nat. Cell Biol. 4(9):674-680 (2002).
Gruer, M.J., et al., Trends Biochem. Sci. 22(1):3-6 (1997).
Klausner, R.D., et al., Mol. Biol. Cell 4(1):1-5 (1993).
Geurts van Kessel, A.H., et al., Cytogenet. Cell Genet. 28(3):169-172 (1980).

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$ 325.00
$ 109.00
Cat# AP1936C
Availability: In Stock
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