|Application ||WB, E|
|Calculated MW||24915 Da|
|Antigen Region||185-214 aa|
|Other Names||39S ribosomal protein L24, mitochondrial, L24mt, MRP-L24, MRPL24|
|Target/Specificity||This MRPL24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-214 amino acids from the C-terminal region of human MRPL24.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MRPL24 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL24 is a 39S subunit protein which is more than twice the size of its E.coli counterpart (EcoL24).
Zhang, Z., et al., Genomics 81(5):468-480 (2003).
O'Brien, T.W., et al., J. Biol. Chem. 275(24):18153-18159 (2000).
Kenmochi, N., et al., Genomics 77 (1-2), 65-70 (2001).
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