MYH14 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
|Application ||WB, IHC-P, E|
|Calculated MW||227871 Da|
|Other Names||Myosin-14, Myosin heavy chain 14, Myosin heavy chain, non-muscle IIc, Non-muscle myosin heavy chain IIc, NMHC II-C, MYH14, KIAA2034|
|Target/Specificity||This MYH14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 654-668 amino acids from the Central region of human MYH14.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MYH14 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.|
|Tissue Location||High levels of expression are found in brain (highest in corpus callosum), heart, kidney, liver, lung, small intestine, colon and skeletal muscle. Expression is low in organs composed mainly of smooth muscle, such as aorta, uterus and urinary bladder. No detectable expression is found in thymus, spleen, placenta and lymphocytes.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping (By similarity).
Leal A.,et al.Gene 312:165-171(2003).
Nagase T.,et al.Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
Grimwood J.,et al.Nature 428:529-535(2004).
Bienvenut W.V.,et al.Submitted (NOV-2006) to UniProtKB.
Jana S.S.,et al.J. Biol. Chem. 284:11563-11571(2009).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.
1 2 3 4 5
1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose