|Application ||WB, E|
|Calculated MW||68685 Da|
|Other Names||Glucokinase regulatory protein, GKRP, Glucokinase regulator, GCKR|
|Target/Specificity||This GCKR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 40-72 amino acids from human GCKR.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GCKR Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6- phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.|
|Cellular Location||Cytoplasm. Nucleus. Note=Under low glucose concentrations, GKRP associates with GCK and the inactive complex is recruited to the hepatocyte nucleus|
|Tissue Location||Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.|
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Provided below are standard protocols that you may find useful for product applications.
Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6- phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.
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Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
de la Iglesia N.,et al.FEBS Lett. 456:332-338(1999).
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