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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EPS15 Antibody (C-term)   

EPS15 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EPS15 Antibody (C-term) AP2159b
    Western blot analysis of anti-EPS15 Pab (Cat. #AP2159b) in HL60 cell line lysate (35ug/lane). EPS15 (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P42566
Other Accession P42567, NP_001972
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 98656 Da
Antigen Region 840-870 aa
Additional Information
Gene ID 2060
Other Names Epidermal growth factor receptor substrate 15, Protein Eps15, Protein AF-1p, EPS15, AF1P
Target/Specificity This EPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 840-870 amino acids from the C-terminal region of human EPS15.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEPS15 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPS15
Synonyms AF1P
Function Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta- 1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit Note=Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin- rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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EPS15 is involved in cell growth regulation, possibly via the regulation of mitogenic signals and control of cell proliferation. EPS15 also participates in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Potential interaction partners include AP2A2, STN2, EPN1, and CRK via its SH3-binding sites. EPS15 is ubiquitously expressed. Phosphorylation on Tyr-849 is involved in the internalization of EGFR. EPS15 is not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis. This protein is involved in a t(1;11)(p32;q23) chromosomal translocation in acute leukemias causing fusion to the trithorax (MLL or HRX) gene product which contains DNA-binding motifs resulting in a rogue activator protein. Structurally, EPS15 contains 2 EF-hand calcium-binding domains, 3 EH domains, and 2 ubiquitin-interacting motif (UIM) repeats.


de Beer, T., et al., Nat. Struct. Biol. 7(11):1018-1022 (2000).
Enmon, J.L., et al., Biochemistry 39(15):4309-4319 (2000).
de Beer, T., et al., Science 281(5381):1357-1360 (1998).
Chen, H., et al., Nature 394(6695):793-797 (1998).
Matsuda, M., et al., J. Biol. Chem. 271(24):14468-14472 (1996).

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$ 325.00
$ 109.00
Cat# AP2159b
Availability: In Stock
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