|Application ||WB, E|
|Calculated MW||14764 Da|
|Antigen Region||20-55 aa|
|Other Names||Protein lgg-1, lgg-1|
|Target/Specificity||This lgg-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-55 amino acids from the center region of CAEEL lgg-1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LGG-1/Atg8 antibodies is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-like modifier involved in autophagy and essential for dauer development and life-span extension (PubMed:12958363, PubMed:20523114). Involved in allophagy, which is an autophagic process in which paternal mitochondria and organelles are degraded during fertilization, and moreover is required for the formation of lgg-2-positive allophagic autophagosomes in embryos (PubMed:24374177). Plays a role in the distribution and clearance of germ cell specific P-granules from somatic cells (PubMed:19167332). Required for normal survival when exposed to pathogenic bacteria S.typhimurium probably by promoting autophagic degradation of intracellular S.typhimurium (PubMed:19667176). Plays a role in mitophagy (PubMed:25896323).|
|Cellular Location||Preautophagosomal structure Cytoplasmic vesicle, autophagosome. Cytoplasmic vesicle, autophagosome membrane. Lysosome lumen. Mitochondrion. Cytoplasm. Note=In embryos, diffuse cytoplasmic localization with some areas displaying a more punctate distribution (PubMed:19377305, PubMed:19167332, PubMed:24374177, PubMed:28806108). Specifically, upon fertilization localizes to autophagosomes around the male pronucleus (PubMed:24374177) During the first embryonic divisions and after the 25-cell stage, localizes to a large population of autophagosomes, with another smaller population of autophagosomes containing both lgg-1 and lgg-2 (PubMed:24374177). Localization to autophagosomes is dependent on atg-7 (PubMed:24374177). Co-localizes with sepa-1 in cytoplasmic aggregates (PubMed:19167332, PubMed:28806108)|
|Tissue Location||Expressed in PLML touch receptor neuron and in the ventral nerve cord.|
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