- CITATIONS: 1
|Application ||WB, IF, E|
|Calculated MW||70384 Da|
|Antigen Region||1-30 aa|
|Other Names||ADP-ribosylation factor-binding protein GGA1, Gamma-adaptin-related protein 1, Golgi-localized, gamma ear-containing, ARF-binding protein 1, GGA1|
|Target/Specificity||This GGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GGA1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GGA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (PubMed:11301005, PubMed:15886016). Mediates export of the GPCR receptor ADRA2B to the cell surface (PubMed:27901063). Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (By similarity). Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (PubMed:15886016, PubMed:15615712).|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein|
|Tissue Location||Ubiquitously expressed.|
Provided below are standard protocols that you may find useful for product applications.
GGA1 is a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.
Zhai, P., et al., Biochemistry 42(47):13901-13908 (2003).
Zhu, G., et al., Biochemistry 42(21):6392-6399 (2003).
Shiba, T., et al., Nat. Struct. Biol. 10(5):386-393 (2003).
Suer, S., et al., Proc. Natl. Acad. Sci. U.S.A. 100(8):4451-4456 (2003).
Shiba, T., et al., Nature 415(6874):937-941 (2002).
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